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KMID : 1138520070100020081
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Journal of Pharmacopuncture
2007 Volume.10 No. 2 p.81 ~ p.86
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Expermental Studies of quantitative evaluation using HPLC and safety of Sweet Bee Venom
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Gok Kyung-Seung
Park Hee-Soo
Kim Min-Ki
Cha Bae-Chun
Lee Eun
Kwon Ki-Rok
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Abstract
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Objectives : This study was conducted to carry out quantitative evaluation and safety of Sweet Bee Venom.
Methods : Content analysis was done using HPLC, measurement of LD$^{50}$ was conducted intravenous, subcutaneous, and intramuscular injection to the ICR mice.
Results : 1. According to HPLC analysis, removal of the enzymes containing phospholipase A2 was successfully rendered on Sweet Bee Venom. And analyzing melittin content, Sweet Bee Venom contained 12% more melittin than Bee Venom. 2. LD$^{50}$ of ICR mice with Sweet Bee Venom was more than 20mg/kg in subcutaneous injection and intravenous injection, between 15mg/kg and 20mg/kg in muscular injection. 3. LD$^{50}$ of ICR mice with Bee Venom was between 6 and 9mg/kg in subcutaneous injection and intravenous injection, and more than 9mg/kg in muscular injection.
Conclusion : Above results indicate that Sweet Bee Venom was more safe than Bee Venom and the process of removing enzymes was well rendered in Sweet Bee Venom.
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KEYWORD
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Sweet Bee Venom, Bee Venom, LD$^{50}$, HPLC analysis, melittin
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